Human AP13(Apelin 13) ELISA Kit

Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AP13 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AP13. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AP13 in the samples is then determined by comparing the OD of the samples to the standard curve.

Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AP13 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AP13. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AP13 in the samples is then determined by comparing the OD of the samples to the standard curve.

Description: serum, plasma or other biological fluids.

Description: A competitive inhibition quantitative ELISA assay kit for detection of Human Apelin 13 (AP13) in samples from serum, plasma or other biological fluids.

Description: A competitive inhibition quantitative ELISA assay kit for detection of Human Apelin 13 (AP13) in samples from serum, plasma or other biological fluids.